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Canine Progesterone Test Kit (Colloidal Gold Immunochromatography)——Scientific Breeding, Precision Monitoring
Canine progesterone (cProg) is an endogenous progestin secreted by the ovarian corpus luteum, playing a vital role in ovulation, pregnancy maintenance, parturition initiation, and mammary gland development in bitches. Accurate progesterone monitoring is a cornerstone of scientific canine reproduction management. CareingBio introduces its rapid canine progesterone test kit based on colloidal gold immunochromatography, providing veterinarians, breeders, and pet owners with an efficient, convenient, and reliable solution for reproductive health monitoring.
I. What Is Canine Progesterone Testing?
Canine progesterone testing measures progesterone concentrations in the bitch‘s blood (serum or plasma), helping determine estrus cycle stages, accurately predict ovulation timing, identify the optimal breeding window, confirm pregnancy, and predict whelping dates. During the estrus cycle, progesterone levels increase predictably. Unlike the transient LH surge, progesterone is easier to track and serves as an ideal marker for assessing estrus progression and ovulation timing.-11
II. Principle of Colloidal Gold Immunochromatography
This kit is based on the competitive colloidal gold immunochromatography method using high-affinity anti-progesterone monoclonal antibodies. Modified progesterone antigen is immobilized on the test line (T-line) of a nitrocellulose (NC) membrane, while anti-progesterone monoclonal antibody-colloidal gold conjugates are pre-dispensed on the conjugate pad. When the test sample is added to the sample well, it migrates forward by capillary action, dissolving the colloidal gold conjugates. Progesterone molecules in the sample bind specifically to the gold-labeled antibodies. As the complexes migrate to the T-line, if progesterone concentration is low, free gold-labeled antibodies bind to the immobilized antigen, producing a visible color. If progesterone concentration is high, progesterone molecules occupy antibody binding sites, inhibiting gold-labeled antibody binding to the T-line antigen, resulting in reduced or absent T-line color. Progesterone levels can be determined qualitatively or semi-quantitatively by visually comparing T-line and control line (C-line) coloration.-1
III. Key Product Advantages
Advantage Description
Rapid & Convenient Visible results within 5–10 minutes; no specialized equipment or training required-7
High Sensitivity Detects progesterone concentrations as low as 1–2 ng/mL, ensuring accuracy even during early estrus-7
High Specificity Based on high-quality monoclonal antibody technology with strong specificity and minimal cross-reactivity
Portable Design Compact test strips ideal for veterinary clinics, breeding facilities, and home environments-7
Cost-Effective Low per-test cost supports frequent monitoring with high value-7
Excellent Stability Stable for 18 months at room temperature (2–30°C) with simple storage requirements-7
IV. Clinical Applications
1. Determine Ovulation Timing for Precise Breeding Window
Serial progesterone monitoring enables accurate determination of ovulation timing. Ovulation occurs approximately 48 hours after the LH peak, with eggs requiring an additional 48 hours to mature. The optimal breeding window is days 4–7 following the LH peak. As the LH peak is narrow and rapidly declines, while progesterone levels rise steadily from the LH peak onward, tracking progesterone changes is the most practical method for identifying the breeding window.-8Optimal breeding progesterone levels vary considerably among individual bitches, typically ranging from 5 to 20 ng/mL. Dynamic monitoring combined with vaginal cytology significantly improves pregnancy rates.-11
2. Confirm Pregnancy and Monitor Gestational Health
Progesterone testing after mating can be used for early pregnancy detection. During normal pregnancy, progesterone concentrations remain elevated, typically维持在15—90 ng/mL. An abnormal drop in progesterone levels may indicate miscarriage risk, requiring timely intervention.-8-26
3. Predict Whelping Time
When progesterone concentration drops below 1 ng/mL, the probability of cervical dilation within 24 hours approaches 100%, serving as an important reference for predicting whelping time and helping breeders prepare adequately for high-risk whelping breeds.-8
4. Aid in Diagnosing Reproductive Disorders
Progesterone testing helps screen for reproductive endocrine disorders such as ovarian cysts and hypoluteoidism, providing critical evidence for infertility diagnosis and management.-7
V. Recommended Testing Protocol
Initial Test Timing: Begin progesterone testing 3–4 days after first observation of vulvar hemorrhagic discharge during the estrus cycle.-8
Testing Frequency: Test every 24–48 hours, with daily testing recommended to capture critical changes.-8
When to Stop: Discontinue testing once the optimal breeding time has been identified.-8
Typically, 4 to 7 progesterone tests are performed throughout the estrus cycle to accurately determine the optimal breeding time.-47
VI. Result Interpretation Guide
Test Result T-line Color Interpretation Recommendation
Negative (Low Progesterone) T-line clearly visible (comparable to C-line) Proestrus or anestrus Continue monitoring
Weak Positive (Rising Progesterone) T-line weaker than C-line Approaching ovulation or early ovulation Retest every 24 hours; prepare for breeding
Positive (High Progesterone) T-line absent or extremely weak Ovulation complete; luteal phase Breeding window may have passed
VII. Product Specifications & Storage
Packaging: 20 tests/kit
Sample Type: Serum or plasma
Assay Time: 5–10 minutes
Storage Conditions: 2–30°C, protected from light and moisture
Shelf Life: 18 months
VIII. About CareingBio
CareingBio is a technology-driven enterprise dedicated to serving life and health, specializing in the R&D, production, and supply of in vitro diagnostic reagents and raw materials. With extensive experience in colloidal gold immunochromatography, ELISA, and other technology platforms, the company has developed proprietary industrial-scale monoclonal antibody pairs, offering customers integrated solutions from raw materials to finished products.-54Guided by the mission of “Serving the Public with Technology, Creating a Healthy Life,” CareingBio is committed to delivering high-quality, reliable products and services for pet health and animal reproduction management.
犬孕酮检测试剂盒（胶体金免疫层析法）——科学繁育，精准护航
犬孕酮（Progesterone, cProg）是一种由卵巢黄体分泌的内源性孕激素，在母犬的排卵、维持妊娠、启动分娩及乳腺发育等生殖活动中发挥着至关重要的作用。精准监测孕酮水平，是科学管理犬繁殖的核心手段。科茵生物推出基于胶体金免疫层析技术的犬孕酮快速检测试剂盒，为兽医、繁育者及宠物主人提供高效、便捷、可靠的生殖健康监测方案。
一、什么是犬孕酮检测？
犬孕酮检测通过测定母犬血液（血清或血浆）中的孕酮浓度，帮助判断发情周期阶段、准确预测排卵时间、确定最佳配种期、确认妊娠状态及预测分娩时间。在母犬发情期间，孕酮浓度呈规律性上升趋势，相较于促黄体生成素（LH）峰值的瞬时出现，孕酮水平更易于追踪，是评估发情进展和排卵时机的理想指标。-11
二、胶体金免疫层析法原理
本试剂盒基于竞争法胶体金免疫层析技术，采用高亲和力抗孕酮单克隆抗体——将改良的孕酮抗原固定在硝酸纤维素膜（NC膜）的检测线（T线）上，抗孕酮单克隆抗体-胶体金标记物预固定在结合垫上。当待检样本滴入加样孔后，通过毛细作用向前层析，溶解结合垫上的胶体金标记物，样本中的孕酮分子与金标抗体特异性结合。当复合物层析至检测线时，若样本中孕酮浓度较低，游离的金标抗体与膜上包被的抗原结合，T线显色；若样本中孕酮浓度较高，孕酮分子占据了抗体结合位点，抑制了金标抗体与T线抗原的结合，T线显色减弱或不显色。通过肉眼观察T线与质控线（C线）的显色情况，即可对孕酮水平作出定性或半定量判断。-1
三、产品核心优势
优势 说明
快速便捷 5—10分钟即可获得肉眼可见结果，无需特殊设备或专业培训，操作简便-7
高灵敏度 可检测低至1—2 ng/mL的孕酮浓度，确保发情早期也能准确检测-7
高特异性 基于优质单克隆抗体技术，特异性强，交叉反应低
携带方便 试纸条小巧轻便，适用于兽医诊所、繁育基地及家庭环境-7
成本经济 单次检测成本低，支持频繁监测，性价比高-7
稳定性好 室温（2—30℃）下可稳定保存18个月，储存条件简便-7
四、临床应用场景
1. 确定排卵时间，精准把握配种窗口
通过连续监测孕酮水平，可精确判定母犬的排卵时间。母犬排卵一般发生在LH峰后约48小时，卵子排出后还需约48小时才能成熟，最佳配种期为LH峰出现后的第4至第7天。由于LH峰窄且消退迅速，而孕酮水平从LH峰值出现后开始持续上升，追踪孕酮变化是定位配种窗口的最实用方法。-8不同犬只的最佳配种孕酮水平差异较大，一般在5—20 ng/mL范围内，采用动态监测与阴道细胞学联合评估可大幅提高受孕率。-11
2. 确认妊娠及监测妊娠健康
交配后检测孕酮水平可用于早期妊娠判断。正常怀孕期间母犬孕酮浓度持续升高，通常维持在15—90 ng/mL。若孕酮水平异常下降，可能提示流产风险，需及时干预。-8-26
3. 预测分娩时间
当孕酮浓度降至1 ng/mL以下时，24小时内宫颈扩张的可能性接近100%，可作为预测分娩时间的重要参考，帮助繁育者为易难产犬种做好充分准备。-8
4. 辅助诊断繁殖障碍
检测孕酮水平可筛查卵巢囊肿、黄体功能不全等生殖内分泌疾病，为不育症诊治提供重要依据。-7
五、推荐检测流程
首次检测时间：在发情周期中首次发现母犬外阴血样分泌物后的第3—4天开始检测孕酮。-8
检测频率：每24—48小时检测一次，推荐每24小时检测一次以确保捕捉到关键变化。-8
结束时机：确定合适的配种时机后即可停止检测。-8
整个发情周期通常需要进行4至7次孕酮检测，以准确锁定最佳繁殖时间。-47
六、结果判读指南
检测结果 T线显色 判读结论 建议
阴性（孕酮低） T线显色明显（与C线相近） 发情前期或乏情期 继续监测
弱阳性（孕酮上升中） T线显色弱于C线 临近排卵或排卵初期 每24小时复测，准备配种
阳性（孕酮高） T线不显色或极弱 排卵完成，进入黄体期 已过最佳配种窗口
七、产品规格与储存
包装规格：20测试/盒
样本类型：血清或血浆
检测时间：5—10分钟
储存条件：2—30℃室温避光干燥保存
有效期：18个月
